Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MAP3K10 / MLK2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117974-100
Catalog Number: LS-G117974
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAP3K10 (NM_002446), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MEKK10 | MKN28 kinase | Protein kinase MST | MAP3K10 | Mixed lineage kinase 2 | MLK2 | MST
- Origin Species
- Human
- NCBI GenBank
- NM_002446
- SwissProt
- Q02779
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 104.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEEEEGAVAK EWGTTPAGPV WTAVFDYEAA GDEELTLRRG DRVQVLSQDC AVSGDEGWWT61GQLPSGRVGV FPSNYVAPGA PAAPAGLQLP QEIPFHELQL EEIIGVGGFG KVYRALWRGE121EVAVKAARLD PEKDPAVTAE QVCQEARLFG ALQHPNIIAL RGACLNPPHL CLVMEYARGG181ALSRVLAGRR VPPHVLVNWA VQVARGMNYL HNDAPVPIIH RDLKSINILI LEAIENHNLA241DTVLKITDFG LAREWHKTTK MSAAGTYAWM APEVIRLSLF SKSSDVWSFG VLLWELLTGE301VPYREIDALA VAYGVAMNKL TLPIPSTCPE PFARLLEECW DPDPHGRPDF GSILKRLEVI361EQSALFQMPL ESFHSLQEDW KLEIQHMFDD LRTKEKELRS REEELLRAAQ EQRFQEEQLR421RREQELAERE MDIVERELHL LMCQLSQEKP RVRKRKGNFK RSRLLKLREG GSHISLPSGF481EHKITVQASP TLDKRKGSDG ASPPASPSII PRLRAIRLTP VDCGGSSSGS SSGGSGTWSR541GGPPKKEELV GGKKKGRTWG PSSTLQKERV GGEERLKGLG EGSKQWSSSA PNLGKSPKHT601PIAPGFASLN EMEEFAEAED GGSSVPPSPY STPSYLSVPL PAEPSPGARA PWEPTPSAPP661ARWGHGARRR CDLALLGCAT LLGAVGLGAD VAEARAADGE EQRRWLDGLF FPRAGRFPRG721LSPPARPHGR REDVGPGLGL APSATLVSLS SVSDCNSTRS LLRSDSDEAA PAAPSPPPSP781PAPTPTPSPS TNPLVDLELE SFKKDPRQSL TPTHVTAACA VSRGHRRTPS DGALGQRGPP841EPAGHGPGPR DLLDFPRLPD PQALFPARRR PPEFPGRPTT LTFAPRPRPA ASRPRLDPWK901LVSFGRTLTI SPPSRPDTPE SPGPPSVQPT LLDMDMEGQN QDSTVPLCGA HGSHSGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
957 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.