Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MAP2K3 / MEK3 / MKK3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102309-100
Catalog Number: LS-G102309
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MEK3(MAP2K3) (NM_145109), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MAP2K3 | MAPKK 3 | MAPKK3 | PRKMK3 | SAPK kinase 2 | SAPKK-2 | SAPKK2 | SKK2 | MAP kinase kinase 3 | MEK 3 | MEK3 | MKK3 | MAPK/ERK kinase 3
- Origin Species
- Human
- NCBI GenBank
- NM_002756
- SwissProt
- P46734
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MESPASSQPA SMPQSKGKSK RKKDLRISCM SKPPAPNPTP PRNLDSRTFI TIGDRNFEVE61ADDLVTISEL GRGAYGVVEK VRHAQSGTIM AVKRIRATVN SQEQKRLLMD LDINMRTVDC121FYTVTFYGAL FREGDVWICM ELMDTSLDKF YRKVLDKNMT IPEDILGEIA VSIVRALEHL181HSKLSVIHRD VKPSNVLINK EGHVKMCDFG ISGYLVDSVA KTMDAGCKPY MAPERINPEL241NQKGYNVKSD VWSLGITMIE MAILRFPYES WGTPFQQLKQ VVEEPSPQLP ADRFSPEFVD301FTAQCLRKNP AERMSYLELM EHPFFTLHKT KKTDIAAFVK EILGEDSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
347 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.