Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MAP2K1 / MKK1 / MEK1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99857-100
Catalog Number: LS-G99857
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MEK1(MAP2K1) (NM_002755), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ERK activator kinase 1 | MAP2K1 | MAPK/ERK kinase 1 | MAPKK 1 | MKK1 | PRKMK1 | MEK 1 | MAP kinase kinase 1 | MAPKK1 | MEK1
- NCBI GenBank
- NM_002755
- SwissProt
- Q02750
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPKKKPTPIQ LNPAPDGSAV NGTSSAETNL EALQKKLEEL ELDEQQRKRL EAFLTQKQKV61GELKDDDFEK ISELGAGNGG VVFKVSHKSS GLVMARKLIH LEIKPAIRNQ IIRELQVLHE121CNSPYIVGFY GAFYSDGEIS ICMEHMDGGS LDQVLKKAGR IPEQILGKVS IAVIKGLTYL181REKHKIMHRD VKPSNILVNS RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY241SVQSDIWSMG LSLVEMAVGR YPIPPPDAKE LELMFGCQVE GDAAETPPRP RTPGRPLSSY301GMDSRPPMAI FELLDYIVNE PPPKLPSGVF SLEFQDFVNK CLIKNPAERA DLKQLMVHAF361IKRSDAEEVD FAGWLCSTIG LNQPSTPTHA AGVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
393 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.