Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MANEA / ENDO Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109780-100
Catalog Number: LS-G109780
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MANEA (NM_024641), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ENDO | Endo-alpha mannosidase | Endomannosidase | HEndo | MANEA | Alpha 1 | 2-endomannosidase | Mandaselin | Mannosidase | endo-alpha
- Origin Species
- Human
- NCBI GenBank
- NM_024641
- SwissProt
- Q5SRI9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAKFRRRTCI ILALFILFIF SLMMGLKMLR PNTATFGAPF GLDLLPELHQ RTIHLGKNFD61FQKSDRINSE TNTKNLKSVE ITMKPSKASE LNLDELPPLN NYLHVFYYSW YGNPQFDGKY121IHWNHPVLEH WDPRIAKNYP QGRHNPPDDI GSSFYPELGS YSSRDPSVIE THMRQMRSAS181IGVLALSWYP PDVNDENGEP TDNLVPTILD KAHKYNLKVT FHIEPYSNRD DQNMYKNVKY241IIDKYGNHPA FYRYKTKTGN ALPMFYVYDS YITKPEKWAN LLTTSGSRSI RNSPYDGLFI301ALLVEEKHKY DILQSGFDGI YTYFATNGFT YGSSHQNWAS LKLFCDKYNL IFIPSVGPGY361IDTSIRPWNT QNTRNRINGK YYEIGLSAAL QTRPSLISIT SFNEWHEGTQ IEKAVPKRTS421NTVYLDYRPH KPGLYLELTR KWSEKYSKER ATYALDRQLP VSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
462 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.