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MAML2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAML2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108817-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAML2 (NM_032427), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MAM2 | MAM3 | Mastermind (Drosophila)-like 2 | MLL-MAML2 | KIAA1819 | MAML2 | Mastermind-like 2 (Drosophila) | MAM-2 | MAM-3 | Mastermind-like protein 2
Origin Species
Human
NCBI GenBank
C91001
SwissProt
Q8IZL2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
125 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGDTAPPQAP AGGLGGASGA GLLGGGSVTP RVHSAIVERL RARIAVCRQH HLSCEGRYER
61GRAESSDRER ESTLQLLSLV QHGQGARKAG KHTKATATAA TTTAPPPPPA APPAASQAAA
121TAAPPPPPDY HHHHQQHLLN SSNNGGSGGI NGEQQPPAST PGDQRNSALI ALQGSLKRKQ
181VVNLSPANSK RPNGFVDNSF LDIKRIRVGE NLSAGQGGLQ INNGQSQIMS GTLPMSQAPL
241RKTNTLPSHT HSPGNGLFNM GLKEVKKEPG ETLSCSKHMD GQMTQENIFP NRYGDDPGEQ
301LMDPELQELF NELTNISVPP MSDLELENMI NATIKQDDPF NIDLGQQSQR STPRPSLPME
361KIVIKSEYSP GLTQGPSGSP QLRPPSAGPA FSMANSALST SSPIPSVPQS QAQPQTGSGA
421SRALPSWQEV SHAQQLKQIA ANRQQHARMQ QHQQQHQPTN WSALPSSAGP SPGPFGQEKI
481PSPSFGQQTF SPQSSPMPGV AGGSGQSKVM ANYMYKAGPS AQGGHLDVLM QQKPQDLSRS
541FINNPHPAME PRQGNTKPLF HFNSDQANQQ MPSVLPSQNK PSLLHYTQQQ QQQQQQQQQQ
601QQQQQQQQQQ QQQQQQQQSS ISAQQQQQQQ SSISAQQQQQ QQQQQQQQQQ QQQQQQQQQQ
661QQPSSQPAQS LPSQPLLRSP LPLQQKLLLQ QMQNQPIAGM GYQVSQQQRQ DQHSVVGQNT
721GPSPSPNPCS NPNTGSGYMN SQQSLLNQQL MGKKQTLQRQ IMEQKQQLLL QQQMLADAEK
781IAPQDQINRH LSRPPPDYKD QRRNVGNMQP TAQYSGGSST ISLNSNQALA NPVSTHTILT
841PNSSLLSTSH GTRMPSLSTA VQNMGMYGNL PCNQPNTYSV TSGMNQLTQQ RNPKQLLANQ
901NNPMMPRPPT LGPSNNNNVA TFGAGSVGNS QQLRPNLTHS MASMPPQRTS NVMITSNTTA
961PNWASQEGTS KQQEALTSAG VRFPTGTPAA YTPNQSLQQA VGSQQFSQRA VAPPNQLTPA
1021VQMRPMNQMS QTLNGQTMGP LRGLNLRPNQ LSTQILPNLN QSGTGLNQSR TGINQPPSLT
1081PSNFPSPNQS SRAFQGTDHS SDLAFDFLSQ QNDNMGPALN SDADFIDSLL KTEPGNDDWM
1141KDINLDEILG NNS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1153 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.