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MAMDC4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAMDC4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G102950-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAMDC4 (NM_206920), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_206920

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
120.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MPLSSHLLPA LVLFLAGSSG WAWVPNHCRS PGQAVCNFVC DCRDCSDEAQ CGYHGASPTL
61GAPFACDFEQ DPCGWRDIST SGYSWLRDRA GAALEGPGPH SDHTLGTDLG WYMAVGTHRG
121KEASTAALRS PTLREAASSC KLRLWYHAAS GDVAELRVEL THGAETLTLW QSTGPWGPGW
181QELAVTTGRI RGDFRVTFSA TRNATHRGAV ALDDLEFWDC GLPTPQANCP PGHHHCQNKV
241CVEPQQLCDG EDNCGDLSDE NPLTCGRHIA TDFETGLGPW NRSEGWSRNH RAGGPERPSW
301PRRDHSRNSA QGSFLVSVAE PGTPAILSSP EFQASGTSNC SLVFYQYLSG SEAGCLQLFL
361QTLGPGAPRA PVLLRRRRGE LGTAWVRDRV DIQSAYPFQI LLAGQTGPGG VVGLDDLILS
421DHCRPVSEVS TLQPLPPGPR APAPQPLPPS SRLQDSCKQG HLACGDLCVP PEQLCDFEEQ
481CAGGEDEQAC GTTDFESPEA GGWEDASVGR LQWRRVSAQE SQGSSAAAAG HFLSLQRAWG
541QLGAEARVLT PLLGPSGPSC ELHLAYYLQS QPREVSCNFE RDTCSWYPGH LSDTHWRWVE
601SRGPDHDHTT GQGHFVLLDP TDPLAWGHSA HLLSRPQVPA APTECLSFWY HLHGPQIGTL
661RLAMRREGEE THLWSRSGTQ GNRWHEAWAT LSHQPGSHAQ YQLLFEGLRD GYHGTMALDD
721VAVRPGPCWA PNYCSFEDSD CGFSPGGQGL WRRQANASGH AAWGPPTDHT TETAQGHYMV
781VDTSPDALPR GQTASLTSKE HRPLAQPACL TFWYHGSLRS PGTLRVYLEE RGRHQVLSLS
841AHGGLAWRLG SMDVQAERAW RVVFEAVAAG VAHSYVALDD LLLQDGPCPQ PGSCDFESGL
901CGWSHLAGPG LGGYSWDWGG GATPSRYPQP PVDHTLGTEA GHFAFFETGV LGPGGRAAWL
961RSEPLPATPA SCLRFWYHMG FPEHFYKGEL KVLLHSAQGQ LAVWGAGGHR RHQWLEAQVE
1021VASAKEFQIV FEATLGGQPA LGPIALDDVE YLAGQHCQQP APSPGNTAAP GSVPAVVGSA
1081LLLLMLLVLL GLGGRRWLQK KGSCPFQSNT EATAPGFDNI LFNADGVTLP ASVTSDP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1137 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.