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MAMDC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAMDC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105847-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAMDC2 (NM_153267), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_153267

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLLRGVLLAL QALQLAGALD LPAGSCAFEE STCGFDSVLA SLPWILNEEG HYIYVDTSFG
61KQGEKAVLLS PDLQAEEWSC LRLVYQITTS SESLSDPSQL NLYMRFEDES FDRLLWSAKE
121PSDSWLIASL DLQNSSKKFK ILIEGVLGQG NTASIALFEI KMTTGYCIEC DFEENHLCGF
181VNRWNPNVNW FVGGGSIRNV HSILPQDHTF KSELGHYMYV DSVYVKHFQE VAQLISPLTT
241APMAGCLSFY YQIQQGNDNV FSLYTRDVAG LYEEIWKADR PGNAAWNLAE VEFNAPYPME
301VIFEVAFNGP KGGYVALDDI SFSPVHCQNQ TELLFSAVEA SCNFEQDLCN FYQDKEGPGW
361TRVKVKPNMY RAGDHTTGLG YYLLANTKFT SQPGYIGRLY GPSLPGNLQY CLRFHYAIYG
421FLKMSDTLAV YIFEENHVVQ EKIWSVLESP RGVWMQAEIT FKKPMPTKVV FMSLCKSFWD
481CGLVALDDIT IQLGSCSSSE KLPPPPGECT FEQDECTFTQ EKRNRSSWHR RRGETPTSYT
541GPKGDHTTGV GYYMYIEASH MVYGQKARLL SRPLRGVSGK HCLTFFYHMY GGGTGLLSVY
601LKKEEDSEES LLWRRRGEQS ISWLRALIEY SCERQHQIIF EAIRGVSIRS DIAIDDVKFQ
661AGPCGEMEDT TQQSSGYSED LNEIEY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

686 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.