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MAGT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAGT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G128936-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAGT1 (NM_032121), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA217H1.1 | Magnesium transporter 1 | IAG2 | OST3B | RP11-217H1.1 | MRX95 | XMEN | IAP | MAGT1 | PRO0756
Origin Species
Human
NCBI GenBank
NM_032121
SwissProt
Q9H0U3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
41.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MRKGKGPICL FSRPTLRPSR SKVSLIEGRG ANMAARWRFW CVSVTMVVAL LIVCDVPSAS
61AQRKKEMVLS EKVSQLMEWT NKRPVIRMNG DKFRRLVKAP PRNYSVIVMF TALQLHRQCV
121VCKQADEEFQ ILANSWRYSS AFTNRIFFAM VDFDEGSDVF QMLNMNSAPT FINFPAKGKP
181KRGDTYELQV RGFSAEQIAR WIADRTDVNI RVIRPPNYAG PLMLGLLLAV IGGLVYLRRS
241NMEFLFNKTG WAFAALCFVL AMTSGQMWNH IRGPPYAHKN PHTGHVNYIH GSSQAQFVAE
301THIVLLFNGG VTLGMVLLCE AATSDMDIGK RKIMCVAGIG LVVLFFSWML SIFRSKYHGY
361PYSFLMS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

367 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.