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MAGI3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAGI3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G105990-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAGI3 (NM_152900), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001142782

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
123.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSKTLKKKKH WLSKVQECAV SWAGPPGDFG AEIRGGAERG EFPYLGRLRE EPGGGTCCVV
61SGKAPSPGDV LLEVNGTPVS GLTNRDTLAV IRHFREPIRL KTVKPGKVIN KDLRHYLSLQ
121FQKGSIDHKL QQVIRDNLYL RTIPCTTRAP RDGEVPGVDY NFISVEQFKA LEESGALLES
181GTYDGNFYGT PKPPAEPSPF QPDPVDQVLF DNEFDAESQR KRTTSVSKME RMDSSLPEEE
241EDEDKEAING SGNAENRERH SESSDWMKTV PSYNQTNSSM DFRNYMMRDE TLEPLPKNWE
301MAYTDTGMIY FIDHNTKTTT WLDPRLCKKA KAPEDCEDGE LPYGWEKIED PQYGTYYVDH
361LNQKTQFENP VEEAKRKKQL GQVEIGSSKP DMEKSHFTRD PSQLKGVLVR ASLKKSTMGF
421GFTIIGGDRP DEFLQVKNVL KDGPAAQDGK IAPGDVIVDI NGNCVLGHTH ADVVQMFQLV
481PVNQYVNLTL CRGYPLPDDS EDPVVDIVAA TPVINGQSLT KGETCMNPQD FKPGAMVLEQ
541NGKSGHTLTG DGLNGPSDAS EQRVSMASSG SSQPELVTIP LIKGPKGFGF AIADSPTGQK
601VKMILDSQWC QGLQKGDIIK EIYHQNVQNL THLQVVEVLK QFPVGADVPL LILRGGPPSP
661TKTAKMKTDK KENAGSLEAI NEPIPQPMPF PPSIIRSGSP KLDPSEVYLK SKTLYEDKPP
721NTKDLDVFLR KQESGFGFRV LGGDGPDQSI YIGAIIPLGA AEKDGRLRAA DELMCIDGIP
781VKGKSHKQVL DLMTTAARNG HVLLTVRRKI FYGEKQPEDD SSQAFISTQN GSPRLNRAEV
841PARPAPQEPY DVVLQRKENE GFGFVILTSK NKPPPGVIPH KIGRVIEGSP ADRCGKLKVG
901DHISAVNGQS IVELSHDNIV QLIKDAGVTV TLTVIAEEEH HGPPSGTNSA RQSPALQHRP
961MGQSQANHIP GDRSALEGEI GKDVSTSYRH SWSDHKHLAQ PDTAVISVVG SRHNQNLGCY
1021PVELERGPRG FGFSLRGGKE YNMGLFILRL AEDGPAIKDG RIHVGDQIVE INGEPTQGIT
1081HTRAIELIQA GGNKVLLLLR PGTGLIPDHG LAPSGLCSYV KPEQH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1125 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.