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MAGI1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAGI1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G120903-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAGI1 (NM_001033057), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AIP-3 | AIP3 | BAIAP1 | BAI1-associated protein 1 | MAGI1 | MAGI-1 | BAP-1 | WW domain-containing protein 3 | TNRC19 | WWP3
Origin Species
Human
NCBI GenBank
NM_004742
SwissProt
Q96QZ7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
161.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSKVIQKKNH WTSRVHECTV KRGPQGELGV TVLGGAEHGE FPYVGAVAAV EAAGLPGGGE
61GPRLGEGELL LEVQGVRVSG LPRYDVLGVI DSCKEAVTFK AVRQGGRLNK DLRHFLNQRF
121QKGSPDHELQ QTIRDNLYRH AVPCTTRSPR EGEVPGVDYN FLTVKEFLDL EQSGTLLEVG
181TYEGNYYGTP KPPSQPVSGK VITTDALHSL QSGSKQSTPK RTKSYNDMQN AGIVHAENEE
241EDDVPEMNSS FTADSGEQEE HTLQETALPP VNSSIIAAPI TDPSQKFPQY LPLSAEDNLG
301PLPENWEMAY TENGEVYFID HNTKTTSWLD PRCLNKQQKP LEECEDDEGV HTEELDSELE
361LPAGWEKIED PVYGIYYVDH INRKTQYENP VLEAKRKKQL EQQQQQQQQQ QQQQQQQQQQ
421QTEEWTEDHS ALVPPVIPNH PPSNPEPARE VPLQGKPFFT RNPSELKGKF IHTKLRKSSR
481GFGFTVVGGD EPDEFLQIKS LVLDGPAALD GKMETGDVIV SVNDTCVLGH THAQVVKIFQ
541SIPIGASVDL ELCRGYPLPF DPDDPNTSLV TSVAILDKEP IIVNGQETYD SPASHSSKTG
601KVNGMKDARP SSPADVASNS SHGYPNDTVS LASSIATQPE LITVHIVKGP MGFGFTIADS
661PGGGGQRVKQ IVDSPRCRGL KEGDLIVEVN KKNVQALTHN QVVDMLVECP KGSEVTLLVQ
721RGGLPVPKKS PKSQPLERKD SQNSSQHSVS SHRSLHTASP SHSTQVLPEF PPAEAQAPDQ
781TDSSGQKKPD PFKIWAQSRS MYENRLPDYQ EQDIFLWRKE TGFGFRILGG NEPGEPIYIG
841HIVPLGAADT DGRLRSGDEL ICVDGTPVIG KSHQLVVQLM QQAAKQGHVN LTVRRKVVFA
901VPKTENEVPS PASSHHSSNQ PASLTEEKRT PQGSQNSLNT VSSGSGSTSG IGSGGGGGSG
961VVSTVVQPYD VEIRRGENEG FGFVIVSSVS RPEAGTTFGN ACVAMPHKIG RIIEGSPADR
1021CGKLKVGDRI LAVNGCSITN KSHSDIVNLI KEAGNTVTLR IIPGDESSNA TLLTNAEKIA
1081TITTTHTPSQ QGTQETRNTT KPKQESQFEF KAPQATQEQD FYTVELERGA KGFGFSLRGG
1141REYNMDLYVL RLAEDGPAER CGKMRIGDEI LEINGETTKN MKHSRAIELI KNGGRRVRLF
1201LKRGDGSVPE YDPSSDRHGP ATGPQGVPEV RAGPDRRQHP SLESSYPPDL HKSSPHGEKR
1261AHARDPKGSR EYSRQPNEHH TWNGTSRKPD SGACRPKDRA PEGRRDAQAE RAAAANGPKR
1321RSPEKRREGT RSADNTLERR EKHEKRRDVS PERRRERSPT RRRDGSPSRR RRSLERLLEQ
1381RRSPERRRGG SPERRAKSTD RRRARSPERR RERSLDKRNR EDRASHRERE EANLKQDAGR
1441SSRHPPEQRR RPYKECSTDL SI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1462 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.