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MAGEE2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAGEE2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107263-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAGEE2 (NM_138703), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MAGE-E2 antigen | MAGEE2 | Melanoma antigen family E | 2 | HCA3 | Melanoma-associated antigen E2
Origin Species
Human
NCBI GenBank
NM_138703
SwissProt
Q8TD90

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLVSQNARH CSAEITADYG DGRGEIQATN ASGSPTSMLV VDAPQCPQAP INSQCVNTSQ
61AVQDPNDLEV LIDEQSRRLG ALRVHDPLED RSIALVNFMR MKSQTEGSIQ QSEMLEFLRE
121YSDQFPEILR RASAHLDQVF GLNLRVIDPQ ADTYNLVSKR GFQITDRIAE SLDMPKASLL
181ALVLGHILLN GNRAREASIW DLLLKVDMWD KPQRINNLFG NTRNLLTTDF VCMRFLEYWP
241VYGTNPLEFE FLWGSRAHRE ITKMEALKFV SDAHDEEPWS WPEEYNKALE GDKTKERSLT
301AGLEFWSEDT MNDKANDLVQ LAISVTEEML PIHQDELLAH TGKEFEDVFP NILNRATLIL
361DMFYGLSLIE VDTSEHIYLL VQQPESEEEQ VMLESLGRPT QEYVMPILGL IFLMGNRVKE
421ANVWNLLRRF SVDVGRKHSI TRKLMRQRYL ECRPLSYSNP VEYELLWGPR AHHETIKMKV
481LEYMARLYRK RPQNWPEQYR EAVEDEEARA KSEATIMFFL DPT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

523 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.