Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MAGED1 / NRAGE Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G122347-100
Catalog Number: LS-G122347
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAGED1 (NM_001005333), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DLXIN-1 | MAGE-D1 antigen | Melanoma antigen family D | 1 | NRAGE | MAGE tumor antigen CCF | MAGED1 | Melanoma-associated antigen D1
- Origin Species
- Human
- NCBI GenBank
- NM_006986
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 91.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAQKMDCGAG LLGFQNPDAC RAVCHPLPQP PASTLPLSAF PTLCDPPYSQ LRDPPAVLSC61YCTPLGASPA PAEASVEDSA LLMQTLMEAI QISEAPPTNQ ATAAASPQSS QPPTANEMAD121IQVSAAAARP KSAFKVQNAT TKGPNGVYDF SQAHNAKDVP NTQPKAAFKS QNATPKGPNA181AYDFSQAATT GELAANKSEM AFKAQNATTK VGPNATYNFS QSLNANDLAN SRPKTPFKAW241NDTTKAPTAD TQTQNVNQAK MATSQADIET DPGISEPDGA TAQTSADGSQ AQNLESRTII301RGKRTRKINN LNVEENSSGD QRRAPLAAGT WRSAPVPVTT QNPPGAPPNV LWQTPLAWQN361PSGWQNQTAR QTPPARQSPP ARQTPPAWQN PVAWQNPVIW PNPVIWQNPV IWPNPIVWPG421PVVWPNPLAW QNPPGWQTPP GWQTPPGWQG PPDWQGPPDW PLPPDWPLPP DWPLPTDWPL481PPDWIPADWP IPPDWQNLRP SPNLRPSPNS RASQNPGAAQ PRDVALLQER ANKLVKYLML541KDYTKVPIKR SEMLRDIIRE YTDVYPEIIE RACFVLEKKF GIQLKEIDKE EHLYILISTP601ESLAGILGTT KDTPKLGLLL VILGVIFMNG NRASEAVLWE ALRKMGLRPG VRHPLLGDLR661KLLTYEFVKQ KYLDYRRVPN SNPPEYEFLW GLRSYHETSK MKVLRFIAEV QKRDPRDWTA721QFMEAADEAL DALDAAAAEA EARAEARTRM GIGDEAVSGP WSWDDIEFEL LTWDEEGDFG781DPWSRIPFTF WARYHQNARS RFPQTFAGPI IGPGGTASAN FAANFGAIGF FWVETRTRPLEQKLISEEDLAANDILDYKDDDDKV
834 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.