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MAGEC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAGEC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G107262-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAGEC3 (NM_138702), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLLPCHWVLD ATFSDGSLGQ WVKNTCATYA LSPVVLPPQP QPRKKATDKD YSAFHLGHLR
61EVRLFLRGGT SDQRMDSLVL CPTYFKLWRT LSGSPGLQLS DLHFGSQPEG KFSLRRAVSV
121KQREEPQDWP LNEKRTLWKD SDLPTWRRGT GYTLSLPAVS PGKRLWGEKA GSLPESEPLF
181TYTLDEKVDK LVQFLLLKYQ AKEPLTRAEM QMNVINTYTG YFPMIFRKAR EFIEILFGIS
241LTEVDPDHFY VFVNTLDLTC EGSLSDEQGM PQNRLLILIL SVIFIKGNCA SEEVIWEVLN
301AIGPWSALAG FADVLSRLAL WESEGPEAFC EESGLRSAEG SVLDLANPQG LAGHRQEDGR
361RGLTEASPQQ KKGGEDEDMP AAGMPPLPQS PPEIPPQGPP KISPQGPPQS PPQSPLDSCS
421SPLLWTRLDE ESSSEEEDTA TWHALPESES LPRYALDEKV AELVQFLLLK YQTKEPVTKA
481EMLTTVIKKY KDYFPMIFGK AHEFIELIFG IALTDMDPDN HSYFFEDTLD LTYEGSLIDD
541QGMPKNCLLI LILSMIFIKG SCVPEEVIWE VLSAIGPIQR PAREVLEFLS KLSSIIPSAF
601PSWYMDALKD MEDRAQAIID TTDDATAMAS ASPSVMSTNF CPE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

643 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.