Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MAGEC2 / CT10 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112765-100
Catalog Number: LS-G112765
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAGEE1(MAGEC2) (NM_016249), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CT10 | Cancer/testis antigen 10 | HCA587 | MAGE-C2 antigen | Melanoma antigen family C | 2 | MAGE-E1 antigen | MAGE-C2 | MAGEC2 | Melanoma-associated antigen C2
- Origin Species
- Human
- NCBI GenBank
- NM_016249
- SwissProt
- Q9UBF1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPPVPGVPFR NADNDSPTSV ELEDWVDAQH PTDEEEEEAS SASSTLYLVF SPSSFSTSSS61LILGGPEEEE VPSGVIPNLT ESIPSSPPQG PPQGPSQSPL SSCCSSFSWS SFSEESSSQK121GEDTGTCQGL PDSESSFTYT LDEKVAELVE FLLLKYEAEE PVTEAEMLMI VIKYKDYFPV181ILKRAREFME LLFGLALIEV GPDHFCVFAN TVGLTDEGSD DEGMPENSLL IIILSVIFIK241GNCASEEVIW EVLNAVGVYA GREHFVYGEP RELLTKVWVQ GHYLEYREVP HSSPPYYEFL301WGPRAHSESI KKKVLEFLAK LNNTVPSSFP SWYKDALKDV EERVQATIDT ADDATVMASE361SLSVMSSNVS FSETRTRPLEQKLISEEDLAANDILDYKDDDDKV
373 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.