Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MAGEA1 / MAGE 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100438-100
Catalog Number: LS-G100438
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAGE 1(MAGEA1) (NM_004988), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Antigen MZ2-E | Cancer/testis antigen 1.1 | CT1.1 | MAGE-1 antigen | MAGE1A | Melanoma antigen family A 1 | Melanoma antigen MAGE-1 | MAGE 1 | MAGE1 | Melanoma-associated antigen 1 | MAGEA1
- Origin Species
- Human
- NCBI GenBank
- NM_004988
- SwissProt
- P43355
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLEQRSLHC KPEEALEAQQ EALGLVCVQA AASSSSPLVL GTLEEVPTAG STDPPQSPQG61ASAFPTTINF TRQRQPSEGS SSREEEGPST SCILESLFRA VITKKVADLV GFLLLKYRAR121EPVTKAEMLE SVIKNYKHCF PEIFGKASES LQLVFGIDVK EADPTGHSYV LVTCLGLSYD181GLLGDNQIMP KTGFLIIVLV MIAMEGGHAP EEEIWEELSV MEVYDGREHS AYGEPRKLLT241QDLVQEKYLE YRQVPDSDPA RYEFLWGPRA LAETSYVKVL EYVIKVSARV RFFFPSLREA301ALREEEEGVT RTRPLEQKLI SEEDLAANDI LDYKDDDDKV
340 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.