Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MAGE12 / MAGEA12 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129604-100
Catalog Number: LS-G129604
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAGEA12 (NM_001166387), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CT1.12 | MAGE-12 antigen | Melanoma antigen family A | 12 | Melanoma-associated antigen 12 | MAGE12 | MAGE12F antigen | Cancer/testis antigen 1.12 | MAGEA12
- Origin Species
- Human
- NCBI GenBank
- NM_005367
- SwissProt
- P43365
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPLEQRSQHC KPEEGLEAQG EALGLVGAQA PATEEQETAS SSSTLVEVTL REVPAAESPS61PPHSPQGAST LPTTINYTLW SQSDEGSSNE EQEGPSTFPD LETSFQVALS RKMAELVHFL121LLKYRAREPF TKAEMLGSVI RNFQDFFPVI FSKASEYLQL VFGIEVVEVV RIGHLYILVT181CLGLSYDGLL GDNQIVPKTG LLIIVLAIIA KEGDCAPEEK IWEELSVLEA SDGREDSVFA241HPRKLLTQDL VQENYLEYRQ VPGSDPACYE FLWGPRALVE TSYVKVLHHL LKISGGPHIS301YPPLHEWAFR EGEETRTRPLEQKLISEEDLAANDILDYKDDDDKV
314 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.