Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MAGE10 / MAGEA10 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121775-100
Catalog Number: LS-G121775
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAGEA10 (NM_001011543), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cancer/testis antigen 1.10 | CT1.10 | MAGE10 | MAGE-10 antigen | Melanoma antigen family A | 10 | MAGEA10 | Melanoma-associated antigen 10
- Origin Species
- Human
- NCBI GenBank
- NM_001011543
- SwissProt
- P43363
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPRAPKRQRC MPEEDLQSQS ETQGLEGAQA PLAVEEDASS STSTSSSFPS SFPSSSSSSS61SSCYPLIPST PEEVSADDET PNPPQSAQIA CSSPSVVASL PLDQSDEGSS SQKEESPSTL121QVLPDSESLP RSEIDEKVTD LVQFLLFKYQ MKEPITKAEI LESVIKNYED HFPLLFSEAS181ECMLLVFGID VKEVDPTGHS FVLVTSLGLT YDGMLSDVQS MPKTGILILI LSIIFIEGYC241TPEEVIWEAL NMMGLYDGME HLIYGEPRKL LTQDWVQENY LEYRQVPGSD PARYEFLWGP301RAHAEIRKMS LLKFLAKVNG SDPRSFPLWY EEALKDEEER AQDRIATTDD TTAMASASSS361ATGSFSYPETRTRPLEQKLISEEDLAANDILDYKDDDDKV
369 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.