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LZTS1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LZTS1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110831-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LZTS1 (NM_021020), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
F37 | LZTS1
Origin Species
Human
NCBI GenBank
NM_021020
SwissProt
Q9Y250

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSVSSLISG HSFHSKHCRA SQYKLRKSSH LKKLNRYSDG LLRFGFSQDS GHGKSSSKMG
61KSEDFFYIKV SQKARGSHHP DYTALSSGDL GGQAGVDFDP STPPKLMPFS NQLEMGSEKG
121AVRPTAFKPV LPRSGAILHS SPESASHQLH PAPPDKPKEQ ELKPGLCSGA LSDSGRNSMS
181SLPTHSTSSS YQLDPLVTPV GPTSRFGGSA HNITQGIVLQ DSNMMSLKAL SFSDGGSKLG
241HSNKADKGPS CVRSPISTDE CSIQELEQKL LEREGALQKL QRSFEEKELA SSLAYEERPR
301RCRDELEGPE PKGGNKLKQA SQKSQRAQQV LHLQVLQLQQ EKRQLRQELE SLMKEQDLLE
361TKLRSYEREK TSFGPALEET QWEVCQKSGE ISLLKQQLKE SQTEVNAKAS EILGLKAQLK
421DTRGKLEGLE LRTQDLEGAL RTKGLELEVC ENELQRKKNE AELLREKVNL LEQELQELRA
481QAALARDMGP PTFPEDVPAL QRELERLRAE LREERQGHDQ MSSGFQHERL VWKEEKEKVI
541QYQKQLQQSY VAMYQRNQRL EKALQQLARG DSAGEPLEVD LEGADIPYED IIATEI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

596 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.