Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LY9 / CD229 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118039-100
Catalog Number: LS-G118039
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CD229(LY9) (NM_002348), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD229 | CD229 antigen | Cell surface molecule Ly-9 | Hly9 | Lymphocyte antigen 9 | MLY9 | SLAMF3 | LY9
- Origin Species
- Human
- NCBI GenBank
- L42621
- SwissProt
- Q9HBG7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 72 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVAPKSHTDD WAPGPFSSKP QRSQLQIFSS VLQTSLLFLL MGLRASGKDS APTVVSGILG61GSVTLPLNIS VDTEIENVIW IGPKNALAFA RPKENVTIMV KSYLGRLDIT KWSYSLCISN121LTLNDAGSYK AQINQRNFEV TTEEEFTLFV YEQLQEPQVT MKSVKVSENF SCNITLMCSV181KGAEKSVLYS WTPREPHASE SNGGSILTVS RTPCDPDLPY ICTAQNPVSQ RSSLPVHVGQ241FCTDPGASRG GTTGETVVGV LGEPVTLPLA LPACRDTEKV VWLFNTSIIS KEREEAATAD301PLIKSRDPYK NRVWVSSQDC SLKISQLKIE DAGPYHAYVC SEASSVTSMT HVTLLIYRRL361RKPKITWSLR HSEDGICRIS LTCSVEDGGN TVMYTWTPLQ KEAVVSQGES HLNVSWRSSE421NHPNLTCTAS NPVSRSSHQF LSENICSGPE RNTKLWIGLF LMVCLLCVGI FSWCIWKRKG481RCSVPAFCSS QAEAPADTPE PTAGHTLYSV LSQGYEKLDT PLRPARQQPT PTSDSSSDSN541LTTEEDEDRP EVHKPISGRY EVFDQVTQEG AGHDPAPEGQ ADYDPVTPYV TEVESVVGEN601TMYAQVFNLQ GKTPVSQKEE SSATIYCSIR KPQVVPPPQQ NDLEIPESPT YENFTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
655 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.