Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LW-1 / HSFX1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112821-100
Catalog Number: LS-G112821
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LW1(HSFX1) (NM_016153), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HSFX1 | LW-1
- Origin Species
- Human
- NCBI GenBank
- NM_016153
- SwissProt
- Q9UBD0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEDKRSLSMA RCEERNSRGQ DHGLERVPFP PQLQSETYLH PADPSPAWDD PGSTGSPNLR61LLTEEIAFQP LAEEASFRRP HPDGDVPPQG EDNLLSLPFP QKLWRLVSSN QFSSIWWDDS121GACRVINQKL FEKEILKRDV AHKVFATTSI KSFFRQLNLY GFRKRRQCTF RTFTRIFSAK181RLVSILNKLE FYCHPYFQRD SPHLLVRMKR RVGVKSAPRH QEEDKPEAAG SCLAPADTEQ241QDHTSPNEND QVTPQHREPA GPNTQIRSGS APPATPVMVP DSAVASDNSP VTQPAGEWSE301GSQAHVTPVA AVPGPAALPF LYVPGSPTQM NSYGPVVALP TASRSTLAMD TTGLPAPGML361PFCHLWVPVT LVAAGAAQPA ASMVMFPHLP ALHHHCPHSH RTSQYMPASD GPQAYPDYAD421QSTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
423 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.