Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LVRN / Laeverin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105308-100
Catalog Number: LS-G105308
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Laeverin(LVRN) (NM_173800), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Aminopeptidase Q | AP-Q | APQ | AQPEP | CHL2 antigen | LVRN | Laeverin
- Origin Species
- Human
- NCBI GenBank
- NM_173800
- SwissProt
- Q6Q4G3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 113.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGPPSSSGFY VSRAVALLLA GLVAALLLAL AVLAALYGHC ERVPPSELPG LRDSEAESSP61PLRQKPTPTP KPSSARELAV TTTPSNWRPP GPWDQLRLPP WLVPLHYDLE LWPQLRPDEL121PAGSLPFTGR VNITVRCTVA TSRLLLHSLF QDCERAEVRG PLSPGTGNAT VGRVPVDDVW181FALDTEYMVL ELSEPLKPGS SYELQLSFSG LVKEDLREGL FLNVYTDQGE RRALLASQLE241PTFARYVFPC FDEPALKATF NITMIHHPSY VALSNMPKLG QSEKEDVNGS KWTVTTFSTT301PHMPTYLVAF VICDYDHVNR TERGKEIRIW ARKDAIANGS ADFALNITGP IFSFLEDLFN361ISYSLPKTDI IALPSFDNHA MENWGLMIFD ESGLLLEPKD QLTEKKTLIS YVVSHEIGHQ421WFGNLVTMNW WNNIWLNEGF ASYFEFEVIN YFNPKLPRNE IFFSNILHNI LREDHALVTR481AVAMKVENFK TSEIQELFDI FTYSKGASMA RMLSCFLNEH LFVSALKSYL KTFSYSNAEQ541DDLWRHFQMA IDDQSTVILP ATIKNIMDSW THQSGFPVIT LNVSTGVMKQ EPFYLENIKN601RTLLTSNDTW IVPILWIKNG TTQPLVWLDQ SSKVFPEMQV SDSDHDWVIL NLNMTGYYRV661NYDKLGWKKL NQQLEKDPKA IPVIHRLQLI DDAFSLSKNN YIEIETALEL TKYLAEEDEI721IVWHTVLVNL VTRDLVSEVN IYDIYSLLKR YLLKRLNLIW NIYSTIIREN VLALQDDYLA781LISLEKLFVT ACWLGLEDCL QLSKELFAKW VDHPENEIPY PIKDVVLCYG IALGSDKEWD841ILLNTYTNTT NKEEKIQLAY AMSCSKDPWI LNRYMEYAIS TSPFTSNETN IIEVVASSEV901GRYVAKDFLV NNWQAVSKRY GTQSLINLIY TIGRTVTTDL QIVELQQFFS NMLEEHQRIR961VHANLQTIKN ENLKNKKLSA RIAAWLRRNTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
990 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.