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LTK Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LTK Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LTK antibodies →
$725.00each
Catalog Number: LS-G118043-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LTK (NM_002344), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LTK | Leukocyte tyrosine kinase | Protein tyrosine kinase 1 | TYK1
Origin Species
Human
NCBI GenBank
NM_002344
SwissProt
P29376

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.68 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGCWGQLLVW FGAAGAILCS SPGSQETFLR SSPLPLASPS PRDPKVSAPP SILEPASPLN
61SPGTEGSWLF STCGASGRHG PTQTQCDGAY AGTSVVVTVG AAGQLRGVQL WRVPGPGQYL
121ISAYGAAGGK GAKNHLSRAH GVFVSAIFSL GLGESLYILV GQQGEDACPG GSPESQLVCL
181GESRAVEEHA AMDGSEGVPG SRRWAGGGGG GGGATYVFRV RAGELEPLLV AAGGGGRAYL
241RPRDRGRTQA SPEKLENRSE APGSGGRGGA AGGGGGWTSR APSPQAGRSL QEGAEGGQGC
301SEAWATLGWA AAGGFGGGGG ACTAGGGGGG YRGGDASETD NLWADGEDGV SFIHPSSELF
361LQPLAVTENH GEVEIRRHLN CSHCPLRDCQ WQAELQLAEC LCPEGMELAV DNVTCMDLHK
421PPGPLVLMVA VVATSTLSLL MVCGVLILVK QKKWQGLQEM RLPSPELELS KLRTSAIRTA
481PNPYYCQVGL GPAQSWPLPP GVTEVSPANV TLLRALGHGA FGEVYEGLVI GLPGDSSPLQ
541VAIKTLPELC SPQDELDFLM EALIISKFRH QNIVRCVGLS LRATPRLILL ELMSGGDMKS
601FLRHSRPHLG QPSPLVMRDL LQLAQDIAQG CHYLEENHFI HRDIAARNCL LSCAGPSRVA
661KIGDFGMARD IYRASYYRRG DRALLPVKWM PPEAFLEGIF TSKTDSWSFG VLLWEIFSLG
721YMPYPGRTNQ EVLDFVVGGG RMDPPRGCPG PVYRIMTQCW QHEPELRPSF ASILERLQYC
781TQDPDVLNSL LPMELGPTPE EEGTSGLGNR SLECLRPPQP QELSPEKLKS WGGSPLGPWL
841SSGLKPLKSR GLQPQNLWNP TYRS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

864 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.