Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LSR / LISCH7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112999-100
Catalog Number: LS-G112999
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Lipolysis Stimulated Lipoprotein Receptor(LSR) (NM_015925), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ILDR3 | LISCH protein | LISCH | Lipolysis-stimulated remnant | LISCH7 | LSR
- Origin Species
- Human
- NCBI GenBank
- NM_015925
- SwissProt
- Q86X29
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQQDGLGVGT RNGSGKGRSV HPSWPWCAPR PLRYFGRDAR ARRAQTAAMA LLAGGLSRGL61GSHPAAAGRD AVVFVWLLLS TWCTAPARAI QVTVSNPYHV VILFQPVTLP CTYQMTSTPT121QPIVIWKYKS FCRDRIADAF SPASVDNQLN AQLAAGNPGY NPYVECQDSV RTVRVVATKQ181GNAVTLGDYY QGRRITITGN ADLTFDQTAW GDSGVYYCSV VSAQDLQGNN EAYAELIVLD241WLFVVVVCLA AFLIFLLLGI CWCQCCPHTC CCYVRCPCCP DKCCCPEALY AAGKAATSGV301PSIYAPSTYA HLSPAKTPPP PAMIPMGPAY NGYPGGYPGD VDRSSSAGGQ GSYVPLLRDT361DSSVASEVRS GYRIQASQQD DSMRVLYYME KELANFDPSR PGPPSGRVER AMSEVTSLHE421DDWRSRPSRG PALTPIRDEE WGGHSPRSPR GWDQEPAREQ AGGGWRARRP RARSVDALDD481LTPPSTAESG SRSPTSNGGR SRAYMPPRSR SRDDLYDQDD SRDFPRSRDP HYDDFRSRER541PPADPRSHHH RTRDPRDNGS RSGDLPYDGR LLEEAVRKKG SEERRRPHKE EEEEAYYPPA601PPPYSETDSQ ASRERRLKKN LALSRESLVVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
630 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.