LSBio
LRWD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRWD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRWD1 antibodies →
$504.00each
Catalog Number: LS-G105986-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRWD1 (NM_152892), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Centromere protein 33 | LRWD1 | ORC-associated protein | ORCA | CENP-33
Origin Species
Human
NCBI GenBank
NM_152892
SwissProt
Q9UFC0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGPLSARLLM QRGRPKSDRL GKIRSLDLSG LELLSEHLDP KLLCRLTQLQ ELDLSNNHLE
61TLPDNLGLSH LRVLRCANNQ LGDVTALCQF PKLEELSLEG NPFLTVNDNL KVSFLLPTLR
121KVNGKDASST YSQVENLNRE LTSRVTAHWE KFMATLGPEE EAEKAQADFV KSAVRDVRYG
181PESLSEFTQW RVRMISEELV AASRTQVQKA NSPEKPPEAG AAHKPRARLA ALKRPDDVPL
241SLSPSKRACA SPSAQVEGSP VAGSDGSQPA VKLEPLHFLQ CHSKNNSPQD LETQLWACAF
301EPAWEEGATS QTVATCGGEA VCVIDCQTGI VLHKYKAPGE EFFSVAWTAL MVVTQAGHKK
361RWSVLAAAGL RGLVRLLHVR AGFCCGVIRA HKKAIATLCF SPAHETHLFT ASYDKRIILW
421DIGVPNQDYE FQASQLLTLD TTSIPLRLCP VASCPDARLL AGCEGGCCCW DVRLDQPQKR
481RVCEVEFVFS EGSEASGRRV DGLAFVNEDI VASKGSGLGT ICLWSWRQTW GGRGSQSTVA
541VVVLARLQWS STELAYFSLS ACPDKGIVLC GDEEGNVWLY DVSNILKQPP LLPAALQAPT
601QILKWPQPWA LGQVVTKTMV NTVVANASFT YLTALTDSNI VAIWGRM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

647 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.