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LRRTM4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRTM4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRTM4 antibodies →
$504.00each
Catalog Number: LS-G125329-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRTM4 (NM_001134745), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LRRTM4
Origin Species
Human
NCBI GenBank
NM_024993
SwissProt
Q86VH4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67 kda
Protein Type
Lysate
Amino Acid Sequence
1MGFHLITQLK GMSVVLVLLP TLLLVMLTGA QRACPKNCRC DGKIVYCESH AFADIPENIS
61GGSQGLSLRF NSIQKLKSNQ FAGLNQLIWL YLDHNYISSV DEDAFQGIRR LKELILSSNK
121ITYLHNKTFH PVPNLRNLDL SYNKLQTLQS EQFKGLRKLI ILHLRSNSLK TVPIRVFQDC
181RNLDFLDLGY NRLRSLSRNA FAGLLKLKEL HLEHNQFSKI NFAHFPRLFN LRSIYLQWNR
241IRSISQGLTW TWSSLHNLDL SGNDIQGIEP GTFKCLPNLQ KLNLDSNKLT NISQETVNAW
301ISLISITLSG NMWECSRSIC PLFYWLKNFK GNKESTMICA GPKHIQGEKV SDAVETYNIC
361SEVQVVNTER SHLVPQTPQK PLIIPRPTIF KPDVTQSTFE TPSPSPGFQI PGAEQEYEHV
421SFHKIIAGSV ALFLSVAMIL LVIYVSWKRY PASMKQLQQH SLMKRRRKKA RESERQMNSP
481LQEYYVDYKP TNSETMDISV NGSGPCTYTI SGSRECEMPH HMKPLPYYSY DQPVIGYCQA
541HQPLHVTKGY ETVSPEQDES PGLELGRDHS FIATIARSAA PAIYLERIAN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

590 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.