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LRRTM3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRTM3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRTM3 antibodies →
$504.00each
Catalog Number: LS-G102490-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRTM3 (NM_178011), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LRRTM3
Origin Species
Human
SwissProt
Q86VH5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGFNVIRLLS GSAVALVIAP TVLLTMLSSA ERGCPKGCRC EGKMVYCESQ KLQEIPSSIS
61AGCLGLSLRY NSLQKLKYNQ FKGLNQLTWL YLDHNHISNI DENAFNGIRR LKELILSSNR
121ISYFLNNTFR PVTNLRNLDL SYNQLHSLGS EQFRGLRKLL SLHLRSNSLR TIPVRIFQDC
181RNLELLDLGY NRIRSLARNV FAGMIRLKEL HLEHNQFSKL NLALFPRLVS LQNLYLQWNK
241ISVIGQTMSW TWSSLQRLDL SGNEIEAFSG PSVFQCVPNL QRLNLDSNKL TFIGQEILDS
301WISLNDISLA GNIWECSRNI CSLVNWLKSF KGLRENTIIC ASPKELQGVN VIDAVKNYSI
361CGKSTTERFD LARALPKPTF KPKLPRPKHE SKPPLPPTVG ATEPGPETDA DAEHISFHKI
421IAGSVALFLS VLVILLVIYV SWKRYPASMK QLQQRSLMRR HRKKKRQSLK QMTPSTQEFY
481VDYKPTNTET SEMLLNGTGP CTYNKSGSRE CEIPLSMNVS TFLAYDQPTI SYCGVHHELL
541SHKSFETNAQ EDTMETHLET ELDLSTITTA GRISDHKQQL A
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

581 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.