Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LRRN4 / C20orf75 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106142-100
Catalog Number: LS-G106142
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRN4 (NM_152611), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DJ1056H1.1 | Leucine rich repeat neuronal 4 | NLRR4 | C20orf75 | LRRN4 | NLRR-4
- Origin Species
- Human
- NCBI GenBank
- NM_152611
- SwissProt
- Q8WUT4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRQTLPLLLL TVLRPSWADP PQEKVPLFRV TQQGPWGSSG SNATDSPCEG LPAADATALT61LANRNLERLP GCLPRTLRSL DASHNLLRAL STSELGHLEQ LQVLTLRHNR IAALRWGPGG121PAGLHTLDLS YNQLAALPPC TGPALSSLRA LALAGNPLRA LQPRAFACFP ALQLLNLSCT181ALGRGAQGGI AEAAFAGEDG APLVTLEVLD LSGTFLERVE SGWIRDLPKL TSLYLRKMPR241LTTLEGDIFK MTPNLQQLDC QDSPALASVA THIFQDTPHL QVLLFQNCNL SSFPPWTLDS301SQVLSINLFG NPLTCSCDLS WLLTDAKRTV LSRAADTMCA PAAGSSGPFS ASLSLSQLPG361VCQSDQSTTL GASHPPCFNR STYAQGTTVA PSAAPATRPA GDQQSVSKAP NVGSRTIAAW421PHSDAREGTA PSTTNSVAGH SNSSVFPRAA STTRTQHRGE HAPELVLEPD ISAASTPLAS481KLLGPFPTSW DRSISSPQPG QRTHATPQAP NPSLSEGEIP VLLLDDYSEE EEGRKEEVGT541PHQDVPCDYH PCKHLQTPCA ELQRRWRCRC PGLSGEDTIP DPPRLQGVTE TTDTSALVHW601CAPNSVVHGY QIRYSAEGWA GNQSVVGVIY ATARQHPLYG LSPGTTYRVC VLAANRAGLS661QPRSSGWRSP CAAFTTKPSF ALLLSGLCAA SGLLLASTVV LSACLCRRGQ TLGLQRCDTH721LVAYKNPAFD DYPLGLQTVSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
740 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.