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LRRN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRN3 antibodies →
$725.00each
Catalog Number: LS-G119108-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRN3 (NM_001099660), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FIGLER5 | Leucine rich repeat neuronal 3 | LRRN3 | NLRR-3 | NLRR3
Origin Species
Human
NCBI GenBank
AL442092
SwissProt
Q9H3W5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MKDMPLRIHV LLGLAITTLV QAVDKKVDCP RLCTCEIRPW FTPRSIYMEA STVDCNDLGL
61LTFPARLPAN TQILLLQTNN IAKIEYSTDF PVNLTSLDLS QNNLSSVTNI NVKKMPQLLS
121VYLEENKLTE LPEKCLSELS NLQELYINHN LLSTISPGAF IGLHNLLRLH LNSNRLQMIN
181SKWFDALPNL EILMIGENPI IRIKDMNFKP LINLRSLVIA GINLTEIPDN ALVGLENLES
241ISFYDNRLIK VPHVALQKVV NLKFLDLNKN PINRIRRGDF SNMLHLKELG INNMPELISI
301DSLAVDNLPD LRKIEATNNP RLSYIHPNAF FRLPKLESLM LNSNALSALY HGTIESLPNL
361KEISIHSNPI RCDCVIRWMN MNKTNIRFME PDSLFCVDPP EFQGQNVRQV HFRDMMEICL
421PLIAPESFPS NLNVEAGSYV SFHCRATAEP QPEIYWITPS GQKLLPNTLT DKFYVHSEGT
481LDINGVTPKE GGLYTCIATN LVGADLKSVM IKVDGSFPQD NNGSLNIKIR DIHANSVLVS
541WKASSKILKS SVKWTAFVKT ENSHAAQSAR IPSDVKVYNL THLNPSTEYK ICIDIPTIYQ
601KNRKKCVNVT TKGLHPDQKE YEKNNTTTLM ACLGGLLGII GVICLISCLS PEMNCDGGHS
661YVRNYLQKPT FALGELYPPL INLWEAGKEK STSLKVKATV IGLPTNMS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

708 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.