Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LRRN2 / GAC1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G103264-100
Catalog Number: LS-G103264
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRN5(LRRN2) (NM_201630), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FIGLER7 | Leucine rich repeat neuronal 2 | GAC1 | LRRN5 | LRRN2 | Leucine rich repeat neuronal 5 | LRANK1
- Origin Species
- Human
- NCBI GenBank
- NM_006338
- SwissProt
- O75325
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRLLVAPLLL AWVAGATAAV PVVPWHVPCP PQCACQIRPW YTPRSSYREA TTVDCNDLFL61TAVPPALPAG TQTLLLQSNS IVRVDQSELG YLANLTELDL SQNSFSDARD CDFHALPQLL121SLHLEENQLT RLEDHSFAGL ASLQELYLNH NQLYRIAPRA FSGLSNLLRL HLNSNLLRAI181DSRWFEMLPN LEILMIGGNK VDAILDMNFR PLANLRSLVL AGMNLREISD YALEGLQSLE241SLSFYDNQLA RVPRRALEQV PGLKFLDLNK NPLQRVGPGD FANMLHLKEL GLNNMEELVS301IDKFALVNLP ELTKLDITNN PRLSFIHPRA FHHLPQMETL MLNNNALSAL HQQTAESLPN361LQEVGLHGNP IRCDCVIRWA NATGTRVRFI EPQSTLCAEP PDLQRLPVRE VPFREMTDHC421LPLISPRSFP PSLQVASGES MVLHCRALAE PEPEIYWVTP AGLRLTPAHA GRRYRVYPEG481TLELRRVTAE EAGLYTCVAQ NLVGADTKTV SVVVGRALLQ PGRDEGQGLE LRVQETHPYH541ILLSWVTPPN TVSTNLTWSS ASSLRGQGAT ALARLPRGTH SYNITRLLQA TEYWACLQVA601FADAHTQLAC VWARTKEATS CHRALGDRPG LIAILALAVL LLAAGLAAHL GTGQPRKGVG661GRRPLPPAWA FWGWSPPSVR VVSAPLVLPW NPGRKLPRSS EGETLLPPLS QNSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
713 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.