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LRRN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRN1 antibodies →
$504.00each
Catalog Number: LS-G110926-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRN1 (NM_020873), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FIGLER3 | KIAA1497 | Leucine rich repeat neuronal 1 | Leucine-rich-repeat protein | LRRN1 | NLRR-1
Origin Species
Human
NCBI GenBank
AI039126
SwissProt
Q6UXK5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARMSFVIAA CQLVLGLLMT SLTESSIQNS ECPQLCVCEI RPWFTPQSTY REATTVDCND
61LRLTRIPSNL SSDTQVLLLQ SNNIAKTVDE LQQLFNLTEL DFSQNNFTNI KEVGLANLTQ
121LTTLHLEENQ ITEMTDYCLQ DLSNLQELYI NHNQISTISA HAFAGLKNLL RLHLNSNKLK
181VIDSRWFDST PNLEILMIGE NPVIGILDMN FKPLANLRSL VLAGMYLTDI PGNALVGLDS
241LESLSFYDNK LVKVPQLALQ KVPSLKFLDL NKNPIHKIQE GDFKNMLRLK ELGINNMGEL
301VSVDRYALDN LPELTKLEAT NNPKLSYIHR LAFRSVPALE SLMLNNNALN AIYQKTVESL
361PNLREISIHS NPLRCDCVIH WINSNKTNIR FMEPLSMFCA MPPEYKGHQV KEVLIQDSSE
421QCLPMISHDS FPNRLNVDIG TTVFLDCRAM AEPEPEIYWV TPIGNKITVE TLSDKYKLSS
481EGTLEISNIQ IEDSGRYTCV AQNVQGADTR VATIKVNGTL LDGTQVLKIY VKQTESHSIL
541VSWKVNSNVM TSNLKWSSAT MKIDNPHITY TARVPVDVHE YNLTHLQPST DYEVCLTVSN
601IHQQTQKSCV NVTTKNAAFA VDISDQETST ALAAVMGSMF AVISLASIAV YFAKRFKRKN
661YHHSLKKYMQ KTSSIPLNEL YPPLINLWEG DSEKDKDGSA DTKPTQVDTS RSYYMW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

716 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.