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LRRIQ4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRIQ4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119334-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRIQ4 (NM_001080460), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LRRIQ4 | LRRC64
Origin Species
Human
NCBI GenBank
NM_001080460
SwissProt
A6NIV6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSKDIKSVEH SPKIHQRNDP QHVNDRTFFI DASNQSLTAI PLEIFTFTEL EEVHLENNQI
61EEIPQEIQRL KNIRVLYLDK NNLRSLCPAL GLLSSLESLD LSYNPIFSSS LVVVSFLHAL
121RELRLYQTDL KEIPVVIFKN LHHLELLGLT GNHLKCLPKE IVNQTKLREI YLKRNQFEVF
181PQELCVLYTL EIIDLDENKI GAIPEEIGHL TGLQKFYMAS NNLPVLPASL CQCSQLSVLD
241LSHNLLHSIP KSFAELRKMT EIGLSGNRLE KVPRLICRWT SLHLLYLGNT GLHRLRGSFR
301CLVNLRFLDL SQNHLHHCPL QICALKNLEV LGLDDNKIGQ LPSELGSLSK LKILGLTGNE
361FLSFPEEVLS LASLEKLYIG QDQGFKLTYV PEHIRKLQSL KELYIENNHL EYLPVSLGSM
421PNLEVLDCRH NLLKQLPDAI CQAQALKELR LEDNLLTHLP ENLDSLVNLK VLTLMDNPME
481EPPKEVCAEG NEAIWKYLKE NRNRNIMATK IQAWWRGTMV QRGFGKFGEL LKPQKKGKTS
541PKDKKGKKDV KGKPGKGKKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

560 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.