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LRRC8E Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRC8E Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRC8E antibodies →
$504.00each
Catalog Number: LS-G109625-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRC8E (NM_025061), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LRRC8E
Origin Species
Human
NCBI GenBank
NM_025061
SwissProt
Q6NSJ5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIPVAEFKQF TEQQPAFKVL KPWWDVLAEY LTVAMLMIGV FGCTLQVTQD KIICLPNHEL
61QENLSEAPCQ QLLPRGIPEQ IGALQEVKGL KNNLDLQQYS FINQLCYETA LHWYTKYFPY
121LVVIHTLIFM VCTSFWFKFP GTSSKIEHFI SILGKCFDSP WTTRALSEVS GENQKGPAAT
181ERAAATIVAM AGTGPGKAGE GEKEKVLAEP EKVVTEPPVV TLLDKKEGEQ AKALFEKVKK
241FRMHVEEGDI LYTMYIRQTV LKVCKFLAIL VYNLVYVEKI SFLVACRVET SEVTGYASFC
301CNHTKAHLFS KLAFCYISFV CIYGLTCIYT LYWLFHRPLK EYSFRSVREE TGMGDIPDVK
361NDFAFMLHLI DQYDSLYSKR FAVFLSEVSE SRLKQLNLNH EWTPEKLRQK LQRNAAGRLE
421LALCMLPGLP DTVFELSEVE SLRLEAICDI TFPPGLSQLV HLQELSLLHS PARLPFSLQV
481FLRDHLKVMR VKCEELREVP LWVFGLRGLE ELHLEGLFPQ ELARAATLES LRELKQLKVL
541SLRSNAGKVP ASVTDVAGHL QRLSLHNDGA RLVALNSLKK LAALRELELV ACGLERIPHA
601VFSLGALQEL DLKDNHLRSI EEILSFQHCR KLVTLRLWHN QIAYVPEHVR KLRSLEQLYL
661SYNKLETLPS QLGLCSGLRL LDVSHNGLHS LPPEVGLLQN LQHLALSYNA LEALPEELFF
721CRKLRTLLLG DNQLSQLSPH VGALRALSRL ELKGNRLEAL PEELGNCGGL KKAGLLVEDT
781LYQGLPAEVR DKMEEE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

796 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.