Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LRRC8D / LRRC5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111905-100
Catalog Number: LS-G111905
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRC8D (NM_018103), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LRRC5 | LRRC8D
- Origin Species
- Human
- NCBI GenBank
- NM_018103
- SwissProt
- Q7L1W4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 98 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFTLAEVASL NDIQPTYRIL KPWWDVFMDY LAVVMLMVAI FAGTMQLTKD QVVCLPVLPS61PVNSKAHTPP GNAEVTTNIP KMEAATNQDQ DGRTTNDISF GTSAVTPDIP LRATYPRTDF121ALPNQEAKKE KKDPTGRKTN LDFQQYVFIN QMCYHLALPW YSKYFPYLAL IHTIILMVSS181NFWFKYPKTC SKVEHFVSIL GKCFESPWTT KALSETACED SEENKQRITG AQTLPKHVST241SSDEGSPSAS TPMINKTGFK FSAEKPVIEV PSMTILDKKD GEQAKALFEK VRKFRAHVED301SDLIYKLYVV QTVIKTAKFI FILCYTANFV NAISFEHVCK PKVEHLIGYE VFECTHNMAY361MLKKLLISYI SIICVYGFIC LYTLFWLFRI PLKEYSFEKV REESSFSDIP DVKNDFAFLL421HMVDQYDQLY SKRFGVFLSE VSENKLREIS LNHEWTFEKL RQHISRNAQD KQELHLFMLS481GVPDAVFDLT DLDVLKLELI PEAKIPAKIS QMTNLQELHL CHCPAKVEQT AFSFLRDHLR541CLHVKFTDVA EIPAWVYLLK NLRELYLIGN LNSENNKMIG LESLRELRHL KILHVKSNLT601KVPSNITDVA PHLTKLVIHN DGTKLLVLNS LKKMMNVAEL ELQNCELERI PHAIFSLSNL661QELDLKSNNI RTIEEIISFQ HLKRLTCLKL WHNKIVTIPP SITHVKNLES LYFSNNKLES721LPVAVFSLQK LRCLDVSYNN ISMIPIEIGL LQNLQHLHIT GNKVDILPKQ LFKCIKLRTL781NLGQNCITSL PEKVGQLSQL TQLELKGNCL DRLPAQLGQC RMLKKSGLVV EDHLFDTLPL841EVKEALNQDI NIPFANGITRTRPLEQKLISEEDLAANDILDYKDDDDKV
858 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.