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LRRC8C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRC8C Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRC8C antibodies →
$725.00each
Catalog Number: LS-G108951-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRC8C (NM_032270), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AD158 | FAD158 | Hypothetical protein AD158 | LRRC8C
Origin Species
Human
NCBI GenBank
NM_032270
SwissProt
Q8TDW0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIPVTEFRQF SEQQPAFRVL KPWWDVFTDY LSVAMLMIGV FGCTLQVMQD KIICLPKRVQ
61PAQNHSSLSN VSQAVASTTP LPPPKPSPAN PITVEMKGLK TDLDLQQYSF INQMCYERAL
121HWYAKYFPYL VLIHTLVFML CSNFWFKFPG SSSKIEHFIS ILGKCFDSPW TTRALSEVSG
181EDSEEKDNRK NNMNRSNTIQ SGPEGSLVNS QSLKSIPEKF VVDKSTAGAL DKKEGEQAKA
241LFEKVKKFRL HVEEGDILYA MYVRQTVLKV IKFLIIIAYN SALVSKVQFT VDCNVDIQDM
301TGYKNFSCNH TMAHLFSKLS FCYLCFVSIY GLTCLYTLYW LFYRSLREYS FEYVRQETGI
361DDIPDVKNDF AFMLHMIDQY DPLYSKRFAV FLSEVSENKL KQLNLNNEWT PDKLRQKLQT
421NAHNRLELPL IMLSGLPDTV FEITELQSLK LEIIKNVMIP ATIAQLDNLQ ELSLHQCSVK
481IHSAALSFLK ENLKVLSVKF DDMRELPPWM YGLRNLEELY LVGSLSHDIS RNVTLESLRD
541LKSLKILSIK SNVSKIPQAV VDVSSHLQKM CIHNDGTKLV MLNNLKKMTN LTELELVHCD
601LERIPHAVFS LLSLQELDLK ENNLKSIEEI VSFQHLRKLT VLKLWHNSIT YIPEHIKKLT
661SLERLSFSHN KIEVLPSHLF LCNKIRYLDL SYNDIRFIPP EIGVLQSLQY FSITCNKVES
721LPDELYFCKK LKTLKIGKNS LSVLSPKIGN LLFLSYLDVK GNHFEILPPE LGDCRALKRA
781GLVVEDALFE TLPSDVREQM KTE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

803 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.