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LRRC8B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRC8B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRC8B antibodies →
$504.00each
Catalog Number: LS-G113280-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRC8B (NM_015350), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0231 | LRRC8B | TALRRP | TA-LRRP
Origin Species
Human
NCBI GenBank
D86984
SwissProt
Q6P9F7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MITLTELKCL ADAQSSYHIL KPWWDVFWYY ITLIMLLVAV LAGALQLTQS RVLCCLPCKV
61EFDNHCAVPW DILKASMNTS SNPGTPLPLP LRIQNDLHRQ QYSYIDAVCY EKQLHWFAKF
121FPYLVLLHTL IFAACSNFWL HYPSTSSRLE HFVAILHKCF DSPWTTRALS ETVAEQSVRP
181LKLSKSKILL SSSGCSADID SGKQSLPYPQ PGLESAGIES PTSSVLDKKE GEQAKAIFEK
241VKRFRMHVEQ KDIIYRVYLK QIIVKVILFV LIITYVPYFL THITLEIDCS VDVQAFTGYK
301RYQCVYSLAE IFKVLASFYV ILVILYGLTS SYSLWWMLRS SLKQYSFEAL REKSNYSDIP
361DVKNDFAFIL HLADQYDPLY SKRFSIFLSE VSENKLKQIN LNNEWTVEKL KSKLVKNAQD
421KIELHLFMLN GLPDNVFELT EMEVLSLELI PEVKLPSAVS QLVNLKELRV YHSSLVVDHP
481ALAFLEENLK ILRLKFTEMG KIPRWVFHLK NLKELYLSGC VLPEQLSTMQ LEGFQDLKNL
541RTLYLKSSLS RIPQVVTDLL PSLQKLSLDN EGSKLVVLNN LKKMVNLKSL ELISCDLERI
601PHSIFSLNNL HELDLRENNL KTVEEIISFQ HLQNLSCLKL WHNNIAYIPA QIGALSNLEQ
661LSLDHNNIEN LPLQLFLCTK LHYLDLSYNH LTFIPEEIQY LSNLQYFAVT NNNIEMLPDG
721LFQCKKLQCL LLGKNSLMNL SPHVGELSNL THLELIGNYL ETLPPELEGC QSLKRNCLIV
781EENLLNTLPL PVTERLQTCL DKC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

803 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.