Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LRRC75B / FAM211B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102723-100
Catalog Number: LS-G102723
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C22orf36(LRRC75B) (NM_207644), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C22orf36 | FAM211B
- Origin Species
- Human
- SwissProt
- Q2VPJ9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGARLGRRAG PEAGSEAGAA AGCGPAPYER RVRWLREIQS TLRERRPERA RQLLRLLRQD61LGLERTLLPD ILYRDVAFLN PVDPISHDLL VNLARDLQCP KKDYELWKSS DKICRQLIYH121LTPHSKQQQG SSLRQRKTQS CLKSSLQKTL LAGETVDLSG IPLSTQDVQH ITRYLSSHGA181VLAVLDLSFT GLSDELLHLL LPSLWALPRL TQLLLNGNRL TRATARKLTD AIKDTTKFPA241LAWVDLGNNV DVASLPQPLL VGLRRRLSQR TSLPTIYEGL DLEPEGSAAG ATTPASTWDS301TAAGLGPEPQ ACCARTRTRPLEQKLISEEDLAANDILDYKDDDDKV
315 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.