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LRRC74A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRC74A Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRC74A antibodies →
$504.00each
Catalog Number: LS-G127623-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C14orf166B(LRRC74A) (NM_194287), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf166B
Origin Species
Human
NCBI GenBank
NM_194287
SwissProt
Q0VAA2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHIQFPSKPT LPRACWEGRI TAGSPGMPPD EIEIEPVRQS SDKMLYCEAE SPPTVEKVKP
61ARENSETDLE IEDDEKFFTT GQKELYLEAC KLMGVVPVSY FIRNMEESYV NLNHHGLGPR
121GTKAIAIALV SNMAVTKLEL EDNCIMEEGV LSLVEMLQEN YYLQEMNISN NHLGLEGARI
181ISDFFERNSS SIWSLELSGN DFKEDSAALL CQALSTNYQI KKLDLSHNQF SDVGGEHLGQ
241MLAINVGLTS LDLSWNNFHT RGAVALCNGL RGNVTLTKLD LSMNGFGNEV ALALGEVLRL
301NRCLVYLDIG GNDIGNEGAS KISKGLESNE SLRVLKLFLN PINMDGAILL ILAIKRNPKS
361RMEELDISNV LVSEQFMKTL DGVYAVHPQL DVVFKAVQGL SPKKTIFLLT NPMKLIQSYA
421DQHKITIVDF FKSLNPTGTM KMSVDEFQKV MIEQNKVPLN QYQVREVIKK LDEKTGMVNF
481SFLNTMKP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

488 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.