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LRRC66 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRC66 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRC66 antibodies →
$725.00each
Catalog Number: LS-G121061-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRC66 (NM_001024611), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LRRC66
Origin Species
Human
NCBI GenBank
NM_001024611
SwissProt
Q68CR7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
97.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKNLYFRVIT IVIGLYFTGI MTNASRKSNI LFNSECQWNE YILTNCSFTG KCDIPVDISQ
61TAATVDVSFN FFRVLLQSHT KKEEWKIKHL DLSNNLISKI TLSPFAYLHA LEVLNLSNNA
121IHSLSLDLLS PKSSWVKRHR SSFRNRFPLL KVLILQRNKL SDTPKGLWKL KSLQSLDLSF
181NGILQIGWSD FHNCLQLENL CLKSNKIFKI PPQAFKDLKK LQVIDLSNNA LITILPMMII
241ALEFPHLVVD LADNNWQCDD SVAVFQNFIS ESWRKKWNVI CNRSIGSEEA NGGTPQSRIS
301RETRLPPIHL HRMKSLIRSK AERPQGGRHT GISTLGKKAK AGSGLRKKQR RLPRSVRSTR
361DVQAAGKKED APQDLALAVC LSVFITFLVA FSLGAFTRPY VDRLWQKKCQ SKSPGLDNAY
421SNEGFYDDME AAGHTPHPET HLRQVFPHLS LYENQTPFWV TQPHPHATVI PDRTLGRSRK
481DPGSSQSPGQ CGDNTGAGSG NDGAVYSILQ RHPHAGNREL MSAAQDHIHR NDILGEWTYE
541TVAQEEPLSA HSVGVSSVAG TSHAVSGSSR YDSNELDPSL SGEITASLCK MLTHAEAQRT
601GDSKERGGTE QSLWDSQMEF SKERQVSSSI DLLSIQQPRL SGARAEEALS AHYSEVPYGD
661PRDTGPSVFP PRWDSGLDVT PANKEPVQKS TPSDTCCELE SDCDSDEGSL FTLSSISSES
721ARSKTEEAVP DEESLQDESS GASKDNVTAV DSLEENVTFQ TIPGKCKNQE DPFEKPLISA
781PDSGMYKTHL ENASDTDRSE GLSPWPRSPG NSPLGDEFPG MFTYDYDTAL QSKAAEWHCS
841LRDLEFSNVD VLQQTPPCSA EVPSDPDKAA FHERDSDILK
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

880 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.