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LRRC56 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRC56 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRC56 antibodies →
$504.00each
Catalog Number: LS-G103854-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRC56 (NM_198075), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LRRC56
Origin Species
Human
NCBI GenBank
AK024495
SwissProt
Q8IYG6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDLGWDRSRG PRRSTSSVRV RELSWQGLHN PCPQSKGPGS QRDRLGEQLV EEYLSPARLQ
61ALARVDDLRL VRTLEMCVDT REGSLGNFGV HLPNLDQLKL NGSHLGSLRD LGTSLGHLQV
121LWLARCGLAD LDGIASLPAL KELYASYNNI SDLSPLCLLE QLEVLDLEGN SVEDLGQVRY
181LQLCPRLAML TLEGNLVCLQ PAPGPTNKVP RGYNYRAEVR KLIPQLQVLD EVPAAHTGPP
241APPRLSQDWL AVKEAIKKGN GLPPLDCPRG APIRRLDPEL SLPETQSRAS RPWPFSLLVR
301GGPLPEGLLS EDLAPEDNTS SLTHGAGQVL CGNPTKGLRE RRHQCQAREP PEQLPQHRPG
361DPAASTSTPE PDPADSSDFL ALAGLRAWRE HGVRPLPYRH PESQQEGAVA PWGPRRVPEE
421QVHQAEPKTP SSPPSLASEP SGTSSQHLVP SPPKHPRPRD SGSSSPRWST DLQSRGRRLR
481VLGSWGPGLG DGVAAVPVLR ALEVASRLSP RAQGCPGPKP APDAAARPPR AAELSHPSPV
541PT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

542 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.