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LRRC4C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRC4C Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRC4C antibodies →
$504.00each
Catalog Number: LS-G110911-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Netrin G1 ligand(LRRC4C) (NM_020929), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1580 | LRRC4C | Netrin-G1 ligand | NGL1 | NGL-1
Origin Species
Human
NCBI GenBank
AB046800
SwissProt
Q9HCJ2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLNKMTLHPQ QIMIGPRFNR ALFDPLLVVL LALQLLVVAG LVRAQTCPSV CSCSNQFSKV
61ICVRKNLREV PDGISTNTRL LNLHENQIQI IKVNSFKHLR HLEILQLSRN HIRTIEIGAF
121NGLANLNTLE LFDNRLTTIP NGAFVYLSKL KELWLRNNPI ESIPSYAFNR IPSLRRLDLG
181ELKRLSYISE GAFEGLSNLR YLNLAMCNLR EIPNLTPLIK LDELDLSGNH LSAIRPGSFQ
241GLMHLQKLWM IQSQIQVIER NAFDNLQSLV EINLAHNNLT LLPHDLFTPL HHLERIHLHH
301NPWNCNCDIL WLSWWIKDMA PSNTACCARC NTPPNLKGRY IGELDQNYFT CYAPVIVEPP
361ADLNVTEGMA AELKCRASTS LTSVSWITPN GTVMTHGAYK VRIAVLSDGT LNFTNVTVQD
421TGMYTCMVSN SVGNTTASAT LNVTAATTTP FSYFSTVTVE TMEPSQDEAR TTDNNVGPTP
481VVDWETTNVT TSLTPQSTRS TEKTFTIPVT DINSGIPGID EVMKTTKIII GCFVAITLMA
541AVMLVIFYKM RKQHHRQNHH APTRTVEIIN VDDEITGDTP MESHLPMPAI EHEHLNHYNS
601YKSPFNHTTT VNTINSIHSS VHEPLLIRMN SKDNVQETQI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

640 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.