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LRRC49 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRC49 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRC49 antibodies →
$504.00each
Catalog Number: LS-G112277-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRC49 (NM_017691), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PGs4 | LRRC49
Origin Species
Human
NCBI GenBank
NM_017691
SwissProt
Q8IUZ0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIPGKYRSVS GRAANNVNCG LHLVIQTSSL PEKNKVEFKL NKDTSSFPGR LLQHDLERNY
61SSRQGDHINL VSSSLSSFPI LQRSSEEKIL YSDRLSLERQ KLTVCPIING EDHLRLLNFQ
121HNFITRIQNI SNLQKLISLD LYDNQIEEIS GLSTLRCLRV LLLGKNRIKK ISNLENLKSL
181DVLDLHGNQI TKIENINHLC ELRVLNLARN FLSHVDNLNG LDSLTELNLR HNQITFVRDV
241DNLPCLQHLF LSFNNISSFD SVSCLADSSS LSDITFDGNP IAQESWYKHT VLQNMMQLRQ
301LDMKRITEEE RRMASVLAKK EEEKKRESHK QSLLKEKKRL TINNVARQWD LQQRVANIAT
361NEDRKDSDSP QDPCQIDGST LSAFPEETGP LDSGLNNALQ GLSVIDTYLV EVDGDTLSLY
421GSGALESLDR NWSVQTAGMI TTVSFTFIEF DEIVQVLPKL KIKFPNSLHL KFKETNLVML
481QQFNALAQLR RIDQLTIDPQ GNPVVNFTLW KYYVLFRLSH FSMQKINGTE VTQNDMIMAE
541RLFGILAHVA SSELPQYRLI SILGDARKKQ FRYLLESKGK KPGIINEENN DSKRLVGENT
601NRATLNYTTR DFYNEKLEEI KEKKKFCKTY IEDLVKEATE INMKNEALQK LWPQMFIELV
661RDAVIEIRNK NSYMKLCLQQ ITDQK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

685 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.