LSBio
LRRC48/DRC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRC48/DRC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRC48/DRC3 antibodies →
$504.00each
Catalog Number: LS-G109261-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRC48(DRC3) (NM_031294), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP586M1120 | LRRC48 | DRC3
Origin Species
Human
NCBI GenBank
NM_031294
SwissProt
Q9H069

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MNQPCNSMEP RVMDDDMLKL AVGDQGPQEE AGQLAKQEGI LFKDVLSLQL DFRNILRIDN
61LWQFENLRKL QLDNNIIEKI EGLENLAHLV WLDLSFNNIE TIEGLDTLVN LEDLSLFNNR
121ISKIDSLDAL VKLQVLSLGN NRIDNMMNII YLRRFKCLRT LSLSRNPISE AEDYKMFICA
181YLPDLMYLDY WRIDDHTKKL AEAKHQYSID ELKHQENLMQ AQLEDEQAQR EELEKHKTAF
241VEHLNGSFLF DSMYAEDSEG NNLSYLPGVG ELLETYKDKF VIICVNIFEY GLKQQEKRKT
301ELDTFSECVR EAIQENQEQG KRKIAKFEEK HLSSLSAIRE ELELPNIEKM ILECSADISE
361LFDALMTLEM QLVEQLEETI NMFERNIVDM VGLFIENVQS LMAQCRDLEN HHHEKLLEIS
421ISTLEKIVEG DLDEDLPNDL RALFVDKDTI VNAVGASHDI HLLKIDNRED ELVTRINSWC
481TRLIDRIHKD EIMRNRKRVK EINQYIDHMQ SELDNLECGD ILD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

523 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.