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LRRC45 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRC45 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRC45 antibodies →
$504.00each
Catalog Number: LS-G106851-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRC45 (NM_144999), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LRRC45
Origin Species
Human
NCBI GenBank
NM_144999
SwissProt
Q96CN5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEEFRRSYSR LCRESGAEPQ EAVLQQLHQL PRGRLDLATQ SLTVETCRAL GKLLPRETLC
61TELVLSDCML SEEGATLLLR GLCANTVLRF LDLKGNNLRA AGAEALGKLL QQNKSIQSLT
121LEWNSLGTWD DAFATFCGGL AANGALQRLD LRNNQISHKG AEELALALKG NTTLQQLDLR
181WNNVGLLGGR ALMNCLPSNR TLWRLDLAGN NIPGDVLRAV EQAMGHSQDR LTTFQENQAR
241THVLSKEVQH LREEKSKQFL DLMETIDKQR EEMAKSSRAS AARVGQLQEA LNERHSIINA
301LKAKLQMTEA ALALSEQKAQ DLGELLATAE QEQLSLSQRQ AKELKLEQQE AAERESKLLR
361DLSAANEKNL LLQNQVDELE RKFRCQQEQL FQTRQEMTSM SAELKMRAIQ AEERLDMEKR
421RCRQSLEDSE SLRIKEVEHM TRHLEESEKA MQERVQRLEA ARLSLEEELS RVKAAALSER
481GQAEEELIKA KSQARLEEQQ RLAHLEDKLR LLAQARDEAQ GACLQQKQVV AEAQTRVSQL
541GLQVEGLRRR LEELQQELSL KDQERVAEVS RVRVELQEQN GRLQAELAAQ EALREKAAAL
601ERQLKVMASD HREALLDRES ENASLREKLR LREAEIARIR DEEAQRASFL QNAVLAYVQA
661SPVRTLSPPK
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

670 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.