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LRRC43 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRC43 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRC43 antibodies →
$725.00each
Catalog Number: LS-G119245-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRC43 (NM_001098519), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LRRC43
Origin Species
Human
NCBI GenBank
NM_001098519
SwissProt
Q8N309

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEASYESESE SESEAGPGTQ RPGTGTVSAA VREHLRKLCL REFPCGAGSW NKSRFLPQTW
61RTWRELVPRE EDVVSPGEET VEALLGLVRS RHSPWALLNN SNAEDSFLRE LAIRNPLTIT
121DTFFYSYFRS LRVIDKKVTL VDKDLLKFLK LEELVLSANR IKEVDATNLP PTLKVLELYG
181NEISSMECLC AHPPAGLQHL GLGHNKLLGP LESLYVTANH WPNLVSLDLG FNDLTDLQSM
241VTSLRTLRHL RLLVLQGNPL ALVPYYRGLT IDSLAQLCVL DDITVSPNEK HLFRGLSLNG
301DLLAQEAQFV VTIGNIRGVL DTSVLDPEPR PEGPFITYNY YVTYDFVKDE EGEMNESAGV
361LAEIVKPSPS LELLVEESPE EVVEDVIEDI VEEVTEEVEG SLESEVEESG ESELSVISGP
421STILQMPRAS AEELAKLRLR IDPRLCPSPG TVLFSTAHKP WAEVIPCSYE MQHSLRDLVP
481LKAFLLAGTT VTIVEEKILS WPVVLPAVDS PLSAKKGKGE KDKKGKEKDR TGKGEKEPAK
541EWKVLKKKKE PPKELRQDPP ILQVLGRGLV ILEPLLAGEP LVSTVCNFGV VRTLTSDRLT
601LARDSKKIKK VAKKEKPKAV IPIYEGDYHP EPLTVEVQIQ LNQCRSAEEA LRMFAV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

656 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.