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LRRC36 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRC36 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRC36 antibodies →
$504.00each
Catalog Number: LS-G111821-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRC36 (NM_018296), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LRRC36 | ROR gamma-binding protein 70 | ROR gamma binding protein 70 | XLHSRF2 | RORBP70 | Heat shock regulated 2
Origin Species
Human
NCBI GenBank
NM_018296

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEQWELDEE GIRRLGALTL EQPELVESLS LQGSYAGKIH SISDAFRNFK NLRSLDLSRN
61LITSLKGIQY LCSLQDLNLY YNNIPSLVEV SRLQPLPFLK ELDLRLNPVV RKDTDYRLFA
121VYTLQTLEKL DDRTVREGER KAAKLHFSQL GNSENFLLEV EKSSREKTMK NCVTGESSAS
181KVSANVDSRI EMDSNKGLFI PFPNREIKDS LSTSATQGNG TRDQKLDTFP LGTQTQEVAR
241REMPSDNHQE DEFRHYSPRQ STVRSPEKMT REGYQVSFLD NKSSGSSPEK ELIPKPDTFH
301LTHDASLSKC LDVGDSSQIH PYQLPSDVGL ENYDSCYSQT LSLHGSLGKR PQRSKNYQEY
361SIKPSNDIKT TASHSCGDLL TSLSNPDSST GRLLKLSSDL YATTHFNSDP AVLVNVEQQL
421STSLDDLTPA HGSVPNNAVL GNRTTPLRTL LLSPGTSEHR KIFTKRSLSP SKRGFKWKDN
481ILANLNLKHG FQDATGSEPL SSDLGSLHGL AGNHSPPISA RTPHVATVLR QLLELVDKHW
541NGSGSLLLNK KFLGPARDLL LSLVVPAPSQ PRCCSHPEDT MKAFCRRELE LKEAAQLVPN
601DMESLKQKLV RVLEENLILS EKIQQLEEGA AISIVSGQQS HTYDDLLHKN QQLTMQVACL
661NQELAQLKKL EKTVAILHES QRSLVVTNEY LLQQLNKEPK GYSGKALLPP EKGHHLGRSS
721PFGKSTLSSS SPVAHETGQY LIQSVLDAAP EPGL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

754 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.