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LRRC32 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRC32 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRC32 antibodies →
$504.00each
Catalog Number: LS-G124872-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRC32 (NM_001128922), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
D11S833E | GARP | Garpin | LRRC32
Origin Species
Human
NCBI GenBank
NM_005512
SwissProt
Q14392

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.98 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRPQILLLLA LLTLGLAAQH QDKVPCKMVD KKVSCQVLGL LQVPSVLPPD TETLDLSGNQ
61LRSILASPLG FYTALRHLDL STNEISFLQP GAFQALTHLE HLSLAHNRLA MATALSAGGL
121GPLPRVTSLD LSGNSLYSGL LERLLGEAPS LHTLSLAENS LTRLTRHTFR DMPALEQLDL
181HSNVLMDIED GAFEGLPRLT HLNLSRNSLT CISDFSLQQL RVLDLSCNSI EAFQTASQPQ
241AEFQLTWLDL RENKLLHFPD LAALPRLIYL NLSNNLIRLP TGPPQDSKGI HAPSEGWSAL
301PLSAPSGNAS GRPLSQLLNL DLSYNEIELI PDSFLEHLTS LCFLNLSRNC LRTFEARRLG
361SLPCLMLLDL SHNALETLEL GARALGSLRT LLLQGNALRD LPPYTFANLA SLQRLNLQGN
421RVSPCGGPDE PGPSGCVAFS GITSLRSLSL VDNEIELLRA GAFLHTPLTE LDLSSNPGLE
481VATGALGGLE ASLEVLALQG NGLMVLQVDL PCFICLKRLN LAENRLSHLP AWTQAVSLEV
541LDLRNNSFSL LPGSAMGGLE TSLRRLYLQG NPLSCCGNGW LAAQLHQGRV DVDATQDLIC
601RFSSQEEVSL SHVRPEDCEK GGLKNINLII ILTFILVSAI LLTTLAACCC VRRQKFNQQY
661KA
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

662 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.