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LRRC31 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRC31 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRC31 antibodies →
$504.00each
Catalog Number: LS-G109814-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRC31 (NM_024727), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LRRC31
Origin Species
Human
NCBI GenBank
NM_024727
SwissProt
Q6UY01

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSQTRKKTSS EGETKPQTST VNKFLRGSNA ESRKEDNDLK TSDSQPSDWI QKTATSETAK
61PLSSEMEWRS SMEKNEHFLQ KLGKKAVNKC LDLNNCGLTT ADMKEMVALL PFLPDLEELD
121ISWNGFVGGT LLSITQQMHL VSKLKILRLG SCRLTTDDVQ ALGEAFEMIP ELEELNLSWN
181SKVGGNLPLI LQKFQKGSKI QMIELVDCSL TSEDGTFLGQ LLPMLQSLEV LDLSINRDIV
241GSLNSIAQGL KSTSNLKVLK LHSCGLSQKS VKILDAAFRY LGELRKLDLS CNKDLGGGFE
301DSPAQLVMLK HLQVLDLHQC SLTADDVMSL TQVIPLLSNL QELDLSANKK MGSSSENLLS
361RLRFLPALKS LVINNCALES ETFTALAEAS VHLSALEVFN LSWNKCVGGN LKLLLETLKL
421SMSLQVLRLS SCSLVTEDVA LLASVIQTGH LAKLQKLDLS YNDSICDAGW TMFCQNVRFL
481KELIELDISL RPSNFRDCGQ WFRHLLYAVT KLPQITEIGM KRWILPASQE EELECFDQDK
541KRSIHFDHGG FQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

552 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.