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LRRC14B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRC14B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRC14B antibodies →
$725.00each
Catalog Number: LS-G119315-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRC14B (NM_001080478), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LRRC14B
Origin Species
Human
NCBI GenBank
NM_001080478
SwissProt
A6NHZ5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDTMRSLRFI SAEALVSHPQ VARQSLDSVA HNLYPLLFKA SYLLEQAEVT RAVLGRWPLE
61EFRLGALLGP GADHPQDLRD RTCRACLEAL VRGLADHVLQ DRSRRRLRVA DLTGIRDVQV
121QRCPCGRALG RWGRTQLLAR TCCELQAEPL AAGRPVEVLA DLFVTEGNFE AVVQALRPAG
181PAPLRVHCPS FRADSLSPSQ LLHVLRLAGP GALRKLEVVH NVRLHAGHVQ QLLAQVGFPR
241LASLTLPTKA FDAPPTYAST PDGEDPLLAS IARELSKMAQ LTELSVAFST LTGKIPTLLG
301PLQTPLRVLD LANCALNHTD MAFLADCAHA AHLEVLDLSG HNLVSLYPST FFRLLSQASR
361TLRILTLEEC GIVDSHVGML ILGLSPCHRL RQLKFLGNPL SARALRRLFT ALCELPELRC
421IEFPVPKDCY PEGAAYPQDE LAMSKFNQQK YDEIAEELRA VLLRADREDI QVSTPLFGSF
481DPDIQETSNE LGAFLLQAFK TALENFSRAL KQIE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

514 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.