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LRRC14 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRRC14 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRRC14 antibodies →
$504.00each
Catalog Number: LS-G113716-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRC14 (NM_014665), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0014 | LRRC14 | LRRC14A
Origin Species
Human
NCBI GenBank
NM_014665
SwissProt
Q15048

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHTLVFLSTR QVLQCQPAAC QALPLLPREL FPLLFKVAFM DKKTVVLREL VHTWPFPLLS
61FQQLLQECAH CSRALLQERP STESMQAVIL GLTARLHTSE PGASTQPLCR KHALRVLDMT
121GLLDDGVEQD PGTMSMWDCT AAVARTCIAQ QQGGAAEPGP APIPVEVRVD LRVNRASYAF
181LREALRSSVG SPLRLCCRDL RAEDLPMRNT VALLQLLDAG CLRRVDLRFN NLGLRGLSVI
241IPHVARFQHL ASLRLHYVHG DSRQPSVDGE DNFRYFLAQM GRFTCLRELS MGSSLLSGRL
301DQLLSTLQSP LESLELAFCA LLPEDLRFLA RSPHAAHLKK LDLSGNDLSG SQLAPFQGLL
361QASAATLLHL ELTECQLADT QLLATLPILT QCASLRYLGL YGNPLSMAGL KELLRDSVAQ
421AELRTVVHPF PVDCYEGLPW PPPASVLLEA SINEEKFARV EAELHQLLLA SGRAHVLWTT
481DIYGRLAADY FSLTRTRPLE QKLISEEDLA ANDILDYKDD DDKV

524 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.