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LRP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRP3 antibodies →
$504.00each
Catalog Number: LS-G118048-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRP3 (NM_002333), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HLRp105 | LRP-3 | LRP3
Origin Species
Human
NCBI GenBank
NM_002333
SwissProt
O75074

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEKRAAAGLE GAPGARAQLA VVCLVNIFLT GRLSSAVPAL AACSGKLEQH TERRGVIYSP
61AWPLNYPPGT NCSWYIQGDR GDMITISFRN FDVEESHQCS LDWLLLGPAA PPRQEAFRLC
121GSAIPPAFIS ARDHVWIFFH SDASSSGQAQ GFRLSYIRGK LGQASCQADE FRCDNGKCLP
181GPWQCNTVDE CGDGSDEGNC SAPASEPPGS LCPGGTFPCS GARSTRCLPV ERRCDGLQDC
241GDGSDEAGCP DLACGRRLGS FYGSFASPDL FGAARGPSDL HCTWLVDTQD SRRVLLQLEL
301RLGYDDYVQV YEGLGERGDR LLQTLSYRSN HRPVSLEAAQ GRLTVAYHAR ARSAGHGFNA
361TYQVKGYCLP WEQPCGSSSD SDGGSLGDQG CFSEPQRCDG WWHCASGRDE QGCPACPPDQ
421YPCEGGSGLC YTPADRCNNQ KSCPDGADEK NCFSCQPGTF HCGTNLCIFE TWRCDGQEDC
481QDGSDEHGCL AAVPRKVITA ALIGSLVCGL LLVIALGCAF KLYSLRTQEY RAFETQMTRL
541EAEFVRREAP PSYGQLIAQG LIPPVEDFPV YSASQASVLQ NLRTAMRRQM RRHASRRGPS
601RRRLGRLWNR LFHRPRAPRG QIPLLTAARP SQTVLGDGFL QPAPGAAPDP PAPLMDTGST
661RAAGDRPPSA PGRAPEVGPS GPPLPSGLRD PECRPVDKDR KVCREPLADG PAPADAPREP
721CSAQDPHPQV STASSTLGPH SPEPLGVCRN PPPPCSPMLE ASDDEALLVC
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

770 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.